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Updated: Aug 30, 2026
Rapid One-step Enzymatic Synthesis and All-aqueous Purification of Trehalose Analogues
Published on: February 17, 2017
Expression, isolation, purification, and biochemical properties of trehalose-6-phosphate hydrolase from
D V Karelov1, V V Krasikov, M A Surjik
1Konstantinov St. Petersburg Institute of Nuclear Physics, Russian Academy of Sciences, Gatchina 188350, Leningrad Region, Russia.
Abstract:
Here we describe cloning, expression, and purification of the enzyme trehalose-6-phosphate hydrolase from thermoresistant strain Bacillus sp. GP16. Principal biochemical properties of the enzyme at different pH and temperature values were determined. Entropy and enthalpy of activation of the enzyme for substrates trehalose-6-phosphate and p-nitrophenyl glucoside were calculated, and the dependence of the kinetic parameters from ionic strength was established.
