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Related Experiment Videos

Caffeine content of specialty coffees.

Rachel R McCusker1, Bruce A Goldberger, Edward J Cone

  • 1Department of Pathology, Immunology, and Laboratory Medicine, University of Florida College of Medicine, P.O. Box 100275, Gainesville, Florida 32610-0275,USA.

Journal of Analytical Toxicology
|November 11, 2003
PubMed
Summary

Specialty coffee caffeine levels vary significantly, even within the same beverage daily. Decaffeinated options contain minimal caffeine, while caffeinated brews show wide concentration ranges.

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Area of Science:

  • Food Science
  • Analytical Chemistry
  • Pharmacology

Background:

  • Caffeine is the most consumed psychoactive drug globally, primarily from coffee.
  • Understanding caffeine content in commercially prepared coffee is crucial for consumers.

Purpose of the Study:

  • To quantify caffeine levels in specialty coffee beverages.
  • To assess variability in caffeine content within caffeinated and decaffeinated samples.

Main Methods:

  • Liquid-liquid extraction was used to isolate caffeine from coffee samples.
  • Gas chromatography with nitrogen-phosphorus detection analyzed caffeine concentrations.

Main Results:

  • Decaffeinated coffees contained less than 17.7 mg/dose.

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  • Caffeinated coffees exhibited a broad range of 58–259 mg/dose.
  • Mean caffeine content was 188 (36) mg per 16-oz cup, with daily variations from 259–564 mg/dose in the same beverage.

    • Conclusions:

    • Significant variability exists in the caffeine content of specialty coffee.
    • Consumers may experience unpredictable caffeine intake from daily coffee consumption.
    • Analytical methods confirm low caffeine in decaffeinated options but high variability in caffeinated ones.