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Related Experiment Videos

Choline availability during embryonic development alters progenitor cell mitosis in developing mouse hippocampus.

Corneliu N Craciunescu1, Craig D Albright, Mei-Heng Mar

  • 1Department of Nutrition, School of Public Health and School of Medicine, University of North Carolina, Chapel Hill, NC 27599-7400, USA.

The Journal of Nutrition
|November 11, 2003
PubMed
Summary

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Dietary choline impacts fetal mouse brain development. Choline deficiency reduces progenitor cell proliferation and increases apoptosis in the developing hippocampus.

Area of Science:

  • Neuroscience
  • Developmental Biology
  • Nutritional Science

Background:

  • Dietary choline is crucial for brain development.
  • Previous studies showed choline's influence on the fetal rat hippocampus.
  • Transgenic mouse models offer new avenues for studying choline's effects.

Purpose of the Study:

  • To investigate the effects of maternal choline availability on fetal mouse brain development.
  • To examine choline's impact on hippocampal progenitor cell proliferation and apoptosis.

Main Methods:

  • Timed-pregnant mice were fed choline-supplemented, control, or choline-deficient diets from embryonic day 12 to 17.
  • Fetal brains were analyzed for phosphocholine and phosphatidylcholine concentrations.
  • Hippocampal progenitor cell proliferation was assessed using phosphorylated histone H3.

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  • Apoptosis was evaluated using morphological analysis, TUNEL assay, and activated caspase-3 immunoreactivity.

    • Main Results:

    • Choline-deficient dams had lower phosphocholine and phosphatidylcholine levels in fetal brains.
    • Fewer proliferating cells were observed in the hippocampus of fetuses from choline-deficient dams.
    • Increased apoptosis was detected in the hippocampus of fetuses from choline-deficient dams.
    • These findings were confirmed by TUNEL and activated caspase-3 assays.

    Conclusions:

    • Maternal dietary choline significantly influences progenitor cell proliferation in the fetal mouse brain.
    • Choline availability affects apoptosis rates in the developing fetal hippocampus.
    • Dietary choline is essential for normal fetal brain development in mice.