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Related Experiment Videos

Homodirectional changes in transcriptome composition and mRNA translation induced by rapamycin and heat shock.

Thomas Preiss1, Julie Baron-Benhamou, Wilhelm Ansorge

  • 1EMBL Heidelberg, Meyerhofstrasse 1, D-69117 Heidelberg, Germany.

Nature Structural Biology
|November 11, 2003
PubMed
Summary

Rapamycin treatment in yeast affects both mRNA levels and translation efficiency. Gene expression changes at the transcriptome level are amplified by coordinated translational regulation, a process termed potentiation.

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Area of Science:

  • Molecular Biology
  • Yeast Genetics
  • Gene Regulation

Background:

  • Cellular transcriptome composition is determined by transcription and mRNA turnover.
  • The transcriptome serves as a template for protein synthesis through translation.
  • Rapamycin, a TOR kinase inhibitor, alters mRNA levels in Saccharomyces cerevisiae.

Purpose of the Study:

  • To simultaneously monitor transcriptome and translational changes in yeast.
  • To investigate the relationship between mRNA levels and translation efficiency under rapamycin treatment.
  • To identify coordinated gene regulation beyond transcriptional control.

Main Methods:

  • DNA microarray analysis was employed to assess genome-wide mRNA levels.
  • Polyribosome association was used to measure translational efficiency.

Related Experiment Videos

  • Comparative analysis of transcriptome and proteome changes was performed.

    • Main Results:

    • Rapamycin treatment induced significant changes in hundreds of yeast genes' mRNA levels.
    • Induced genes showed correlated increases in translational efficiency.
    • Reduced mRNA levels were associated with decreased translational fitness.
    • Heat-shock experiments also revealed coordinated transcriptional and translational responses.

    Conclusions:

    • Signal-induced changes in the transcriptome are amplified at the translational level.
    • A coordinated gene regulation mechanism, termed 'potentiation,' operates beyond transcriptional control.
    • This study reveals a higher level of gene regulation coordinating mRNA abundance and translation.