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Bronchoalveolar eosinophilia in random-source versus purpose-bred dogs
L J Baudendistel1, G A Vogler, P A Frank
1Department of Anesthesiology, St. Louis University School of Medicine, MO 63110.
Laboratory Animal Science
|October 1, 1992
Summary
Random-source dogs exhibit elevated eosinophils (EOS) and thromboxane B2 (TxB2) in bronchoalveolar lavage fluid, suggesting a link between airway inflammation and vascular reactivity. This profile persisted despite treatment.
Area of Science:
- Veterinary Medicine
- Pulmonary Physiology
- Immunology
Background:
- Eosinophils (EOS) are implicated in airway and vascular reactivity.
- Previous studies suggest a role for eosinophils in various disease states.
Purpose of the Study:
- To compare bronchoalveolar lavage fluid (BALF) cellular profiles and mediator levels between random-source (RS) and purpose-bred (PB) dogs.
- To investigate the relationship between eosinophils, thromboxane B2 (TxB2), and physiological parameters in dogs.
Main Methods:
- Analysis of BALF cells and mediators (TxB2, 6-keto-PGF1a) from RS and PB dogs.
- Assessment of physiological parameters including heart rate and cardiac output.
- Regression analysis to correlate EOS numbers with TxB2 levels.
Main Results:
- RS dogs had significantly higher total nucleated cell counts and percentage of EOS in BALF compared to PB dogs.
- BALF TxB2 levels were significantly elevated in RS dogs, correlating strongly with absolute EOS numbers.
- RS dogs exhibited increased heart rates and cardiac output under baseline conditions.
Conclusions:
- Elevated eosinophils and thromboxane B2 in BALF of RS dogs suggest a link to airway inflammation and altered vascular reactivity.
- The observed cellular profile and mediator levels persisted despite anthelminthic treatment, indicating a chronic inflammatory state.
- Findings highlight potential differences in pulmonary and cardiovascular physiology between RS and PB dogs.