Related Experiment Video
Updated: Aug 30, 2026

Isolation, Propagation, and Identification of Bacterial Species with Hydrocarbon Metabolizing Properties from Aquatic Habitats
Published on: December 7, 2021
[Viability of heterotrophic bacteria in the Bay of Marseilles]
Gérald Grégori1, Michel Denis, Dominique Lefèvre
1Purdue University Cytometry Laboratories, Hansen Life Sciences Research Building, Room B050, 201 S. University Street, West Lafayette, IN 47907-2064, USA.
Abstract:
Marine microorganism activities are commonly assessed by bulk methods and assigned to the total cell count. The presence in significant amounts of ghost, dead, and damaged cells makes such as assignment a non-correct one. A Nucleic Acid Double Staining protocol (NADS) of fresh water bacteria (Barbesti et al., Cytometry 40 (2000) 214-218) has been adapted to resolve viable, damaged and dead cells in marine environments (Grégori et al., Appl. Environ. Microbiol. 67 (2001) 4662-4670). The present reports the first in situ application of this approach, conducted in the Bay of Marseilles in winter and spring periods at two sites with contrasted features.
Related Concept Videos
Marine Microbial Ecology
Deep Sea Microbial Ecology
Microbial Mats
Microbial Wastewater Treatment
Microbial Interactions: Competition
Microbial Bioremediation of Hydrocarbons

