Related Experiment Video
Updated: Jun 19, 2026

Development of Cell-type specific anti-HIV gp120 aptamers for siRNA delivery
Published on: June 23, 2011
Epigallocatechin gallate, the main component of tea polyphenol, binds to CD4 and interferes with gp120 binding
Kazushige Kawai1, Nelson H Tsuno, Joji Kitayama
1Department of Surgical Oncology, Faculty of Medicine, the University of Tokyo, Japan.
Background:
Epigallocatechin gallate (EGCG), the major component of tea polyphenol, has been reported to have various physiologic modulatory activities. Several reports also have shown that catechin has a protective effect against HIV infection, part of which is mediated by inhibiting virions to bind to the target cell surface.
Objective:
We investigated the effect of EGCG on the expression of CD4 molecules and on its ability to bind gp120, an envelope protein of HIV-1.
Methods:
Peripheral blood CD4+ T cells were incubated in the presence of EGCG, and the expression of CD4 was evaluated by means of flow cytometry. The effect of EGCG on the antibody binding to CD4 was investigated by using a sandwich ELISA, and the effect on the gp120 binding to CD4 was analyzed by means of flow cytometry.
Results:
EGCG efficiently inhibited binding of anti-CD4 antibody to its corresponding antigen. This effect was mediated by the direct binding of EGCG to the CD4 molecule, with consequent inhibition of antibody binding, as well as gp120 binding.
Conclusion:
The present results suggest a potential preventive effect of EGCG on HIV-1 infection by modulating binding to CD4.
Related Concept Videos
Eukaryotic Transcription Inhibitors
Eukaryotic transcription inhibitors usually contain two distinct domains, a DNA...
Inhibition of Cdk Activity
GPCRs Regulate Adenylyl Cylase Activity
Two...
Inhibitors of Virion Maturation and Assembly

