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Bacterial Transformation

In 1928, bacteriologist Frederick Griffith worked on a vaccine for pneumonia, which is caused by Streptococcus pneumoniae bacteria. Griffith studied two pneumonia strains in mice: one pathogenic and one non-pathogenic. Only the pathogenic strain killed host mice.Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...
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Agrobacterium tumefaciens and Agrobacterium rhizogenes-Mediated Transformation of Potato and the Promoter Activity of a Suberin Gene by GUS Staining
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Agrobacterium tumefaciens and Agrobacterium rhizogenes-Mediated Transformation of Potato and the Promoter Activity of a Suberin Gene by GUS Staining

Published on: March 29, 2019

Efficient and genotype-independent Agrobacterium--mediated tomato transformation.

Sung Hun Park1, Jay L Morris, Jung Eun Park

  • 1Department of Horticultural Sciences, Texas A&M University, Vegetable & Fruit Improvement Center, College Station, TX 77845, USA. s-park4@tamu.edu

Journal of Plant Physiology
|November 13, 2003
PubMed
Summary
This summary is machine-generated.

A new Agrobacterium-mediated transformation method efficiently transforms five tomato (Lycopersicon esculentum) cultivars. This simple protocol achieves over 20% transformation frequency without feeder layers, enabling stable transgene integration.

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Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes
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Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes

Published on: February 14, 2020

Area of Science:

  • Plant Biotechnology
  • Molecular Biology
  • Genetics

Background:

  • Efficiently transforming tomato (Lycopersicon esculentum) is crucial for crop improvement.
  • Previous transformation methods often involve complex procedures or feeder layers, limiting their general applicability.

Purpose of the Study:

  • To develop a simple, efficient, and generalizable method for Agrobacterium-mediated transformation of multiple tomato cultivars.
  • To compare the efficacy of different explants and regeneration media with and without Agrobacterium cocultivation.

Main Methods:

  • Explants (leaf, cotyledon, hypocotyl) from five tomato cultivars were cultured on various media.
  • Agrobacterium tumefaciens cocultivation was performed on specific media formulations.
  • Transformation efficiency was assessed, and stable transgene integration was confirmed by Southern blot analysis.

Main Results:

  • Cocultivation with Agrobacterium tumefaciens significantly reduced callus induction and regeneration.
  • A refined protocol using hypocotyl or cotyledon explants achieved >20% transformation frequency across all five cultivars.
  • The optimized method did not require feeder layers and used minimal subculture media.

Conclusions:

  • A highly efficient and simplified Agrobacterium-mediated transformation protocol for tomato (Lycopersicon esculentum) has been established.
  • This method demonstrates broad applicability across different tomato cultivars.
  • The protocol facilitates stable transgene integration and transmission, offering a valuable tool for tomato genetic research and breeding.