Two-dimensional correlation analysis in application to a kinetic model of parallel reactions
Related Experiment Video
Updated: Aug 30, 2026

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells
Published on: December 11, 2021
Thou-Long Chin1, King-Chuen Lin
1Department of Chemistry, National Taiwan University, Institute of Atomic and Molecular Sciences, Academia Sinica, Taipei 106, Taiwan, Republic of China.
Abstract:
By applying generalized two-dimensional (2D) correlation analysis as reported by Noda, we have systematically studied a kinetic model of parallel reactions. Given the related rate constants and absorption coefficients, the correlation between reactant and products are analyzed. The reactant-reactant, reactant-product, and product-product pairs are found to be synchronously correlated, and their intensities increase with increase of the rate constant and the absorption coefficient. On the other hand, only the reactant-product pairs show in the asynchronous spectra. Their intensities also depend proportionally on the rate constant and the absorption coefficient. The influence of signal-to-noise ratio (S/N) and overlapped spectra are further discussed. The resulting synchronous and asynchronous correlation spectra for the kinetic model appear to be weakly influenced by poor quality of the signal when the reference spectrum is set at zero. The ratio of asynchronous to synchronous correlation intensity yields a coherence spectrum. This spectrum remains a constant intensity for all the correlated peaks, being free from the influence of rate constant and absorption coefficient as well as being weakly disturbed by a small S/N ratio. It also provides a way to evaluate the extent of spectral overlap between two peaks. The coherence spectrum is useful to characterize the type of parallel reactions.
Related Concept Videos
Correlation of Experimental Data
For example, a spherical particle moving through a viscous fluid experiences drag. Dimensional analysis shows that the drag force depends on the particle's diameter, velocity, and...
2D NMR: Overview of Heteronuclear Correlation Techniques
2D NMR: Overview of Homonuclear Correlation Techniques
COSY90 is the standard two-dimensional (2D) COSY experiment that...
One-Compartment Open Model: Wagner-Nelson and Loo Riegelman Method for ka Estimation
On...
Reaction Mechanisms: The Steady-State Approximation
Reaction Mechanisms: Rate-limiting Step Approximation

