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Updated: Aug 30, 2026

Isolation and Functional Analysis of Mitochondria from Cultured Cells and Mouse Tissue
Published on: March 23, 2015
Alterations in mitochondria function and morphology in HT29 cells upon conditions inducing differentiation and
Vlasta Sovová1, Jitka Stokrová, Eva Sloncová
1Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, 166 37 Praha 6, Czech Republic.
Abstract:
Morphological and biochemical studies of HT29 cells treated with sodium butyrate and/or glucose-deprived revealed both apoptotic and differentiation response. The main apoptotic response was accompanied with an increase of floating cells. However, the ultrastructural analysis of adherent cells showed the typical apoptotic character of the nucleus in some of them. In addition, remarkable changes of mitochondria, assumed as early stages starting the apoptotic cascade, were observed. These changes were represented not only by alterations of mitochondrial morphology, but also by the number of mitochondria and their localization.
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