Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Poly(dimethylsiloxane)-based microfluidic device with electrospray ionization-mass spectrometry interface for protein

Wang-Chou Sung1, Sheng-Yu Huang, Pao-Chi Liao

  • 1Department of Chemistry, National Cheng Kung University, Tainan City, Taiwan.

Electrophoresis
|November 13, 2003
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cardiac extracellular vesicle proteomics identifies mitochondrial and contractile dysfunction in carbon monoxide poisoning and their reversal by hyperbaric oxygen therapy.

Molecular medicine (Cambridge, Mass.)·2026
Same author

Comprehensive Profiling of Human Urinary Mercapturic Acid Conjugates Associated with Exposure to Reactive Chemical Species Using Enzymatic Deacetylation and High-Resolution Mass Spectrometry.

Analytical chemistry·2026
Same author

Enhanced Environmental PFAS Characterization Using a Virtual High-Resolution Mass Spectral Library Generated by Transfer Learning-Based Neural Network.

Environmental science & technology·2026
Same author

Integrated circuit-microfluidic biosensors for blood-based disease diagnostics-A review.

Analytica chimica acta·2026
Same author

Integrating liquid chromatography-high resolution mass spectrometry based suspect screening with ionization efficiency prediction to expand semi-quantitative coverage in wastewater-based epidemiology: application to environmental chemicals.

Environment international·2026
Same author

m6A modification-mediated lncRNA UNC5B-AS1 enhances HCC progression through miR-760/IL6 axis.

Scientific reports·2026

This study presents an easy method for fabricating poly(dimethylsiloxane) microfluidic chips for protein identification. The modified microchip offers stable electroosmotic flow and efficient analysis via mass spectrometry.

Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Microfluidics

Background:

  • Microfluidic devices offer miniaturization and integration for complex biological analyses.
  • Poly(dimethylsiloxane) (PDMS) is a common material for microfluidic chip fabrication.
  • Stable and reproducible separations are crucial for sensitive analytical techniques like mass spectrometry.

Purpose of the Study:

  • To develop an easy method for fabricating PDMS-based microfluidic chips for protein identification.
  • To enhance the stability of electroosmotic flow in microchannels for improved separation.
  • To demonstrate the feasibility of the integrated system for protein analysis using tandem mass spectrometry.

Main Methods:

  • Fabrication of PDMS microfluidic chips with electrophoretic microchannels and a sheathless nanoelectrospray ionization (ESI) interface.

Related Experiment Videos

  • Surface modification of microchannels with 2-acrylamido-2-methyl-1-propanesulfonic acid (AMPS).
  • Flow-through sampling, capillary electrophoresis separation, and ESI-tandem mass spectrometry (MS/MS) analysis of tryptic peptides and a model protein.

    • Main Results:

    • Stable electroosmotic flow was achieved in AMPS-modified microchannels under acidic buffer conditions.
    • Successful separation and identification of three tryptic peptides and their acetylated products from a standard mixture.
    • Demonstrated compatibility with upstream miniaturized enzymatic digestion and desalting cartridges for integrated protein analysis.

    Conclusions:

    • The developed PDMS microfluidic chip provides a stable and efficient platform for protein identification.
    • The AMPS surface modification significantly improves electroosmotic flow stability.
    • The integrated system shows promise for streamlined and automated proteomic analyses.