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Microdevice for separation and quantitative fraction collection.

Michal Spesný1, Frantisek Foret

  • 1Institute of Analytical Chemistry, Czech Academy of Sciences, Brno, Czech Republic.

Electrophoresis
|November 13, 2003
PubMed
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A novel microfluidic device enables quantitative whole-column fraction collection for capillary electrophoresis. This CD-like system uses centrifugal force for precise separation and collection of sample zones.

Area of Science:

  • Analytical Chemistry
  • Microfluidics
  • Separation Science

Background:

  • Quantitative whole-column fraction collection is crucial for analyzing complex samples.
  • Existing methods for fraction collection in electrophoresis can be cumbersome and lack precision.

Purpose of the Study:

  • To develop a novel microfluidic device for quantitative whole-column fraction collection in electrophoresis.
  • To integrate electrophoretic separation and fraction collection within a single microdevice.

Main Methods:

  • Fabrication of a microfluidic device on a polycarbonate disk using injection molding.
  • Design of a CD-like format utilizing centrifugal force for liquid manipulation.
  • Integration of serpentine separation channels with distributed fraction collection reservoirs.

Related Experiment Videos

  • Testing with visual monitoring of isotachophoretic separation and collection of cationic dyes.

    • Main Results:

    • A prototype microfluidic device was successfully fabricated.
    • The device demonstrated the capability for quantitative whole-column fraction collection.
    • Centrifugal force effectively controlled liquid movement within the microchannels.
    • Isotachophoretic separation and collection of cationic dyes were visually confirmed.

    Conclusions:

    • The developed microfluidic concept offers a promising approach for precise electrophoretic fraction collection.
    • The CD-like format and centrifugal force integration provide a compact and efficient separation system.
    • This technology has potential applications in various analytical chemistry fields requiring sample fractionation.