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Related Experiment Videos

Characterizing embryonic gene expression patterns in the mouse using nonredundant sequence-based selection.

Rita Sousa-Nunes1, Amer Ahmed Rana, Ross Kettleborough

  • 1Division of Mammalian Development, National Institute for Medical Research, The Ridgeway, London NW7 1AA, United Kingdom.

Genome Research
|November 14, 2003
PubMed
Summary
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Researchers studied gene expression in early mouse development, identifying novel genes and their specific expression patterns in the endoderm. This research aids understanding of embryonic tissue patterning and axial specification.

Area of Science:

  • Developmental Biology
  • Genomics
  • Mouse Embryology

Background:

  • Early mouse development involves complex gene expression for tissue patterning.
  • The endoderm is crucial for axial specification and initial tissue formation.
  • Understanding gene expression in these early stages is vital for developmental biology.

Purpose of the Study:

  • To investigate the expression patterns of genes in the early mouse embryo.
  • To identify novel genes involved in endoderm development.
  • To characterize the spatial expression of genes within the 7.5 days postcoitum (dpc) endoderm.

Main Methods:

  • Isolation of cDNAs from 7.5 dpc mouse endoderm.
  • Sequencing of 1978 cDNA clones to identify distinct genes.

Related Experiment Videos

  • In situ hybridization analysis of 160 selected cDNAs.

    • Main Results:

    • 1440 distinct cDNAs were identified from the library.
    • 123 of these cDNAs represented novel genes in the mouse.
    • 18% (29 out of 160) of analyzed genes showed restricted expression patterns.

    Conclusions:

    • A significant number of novel genes are expressed during early mouse endoderm development.
    • Restricted expression patterns suggest specific roles in tissue patterning and axial specification.
    • This study provides a valuable dataset for further research into mouse embryogenesis.