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Updated: Aug 30, 2026

Isolation, Culture, and Characterization of Prostate Cancer-Associated Fibroblasts
Published on: August 1, 2025
Androgen-dependent expression of c-jun and c-fos in human non-transformed epithelial prostatic cells: association
Ilma S Brum1, Débora M Morsch, Adriane Pozzobon
1Department of Physiology, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil. ilma@vortex.ufrgs.br
Objective:
To assess the effect of dihydrotestosterone (DHT) on the gene expression of C-FOS and C-JUN and on the proliferation of human non-transformed epithelial prostatic (HNTEP) cells.
Methods:
Cell proliferation (MTT) and C-FOS and C-JUN mRNA expression (RT-PCR) were determined in cells treated with DHT (10(-8), 10(-10), and 10(-13)M) or with control medium.
Results:
DHT 10(-13) M had a significant stimulatory effect on cell proliferation (p < 0.05) and C-FOS and C-JUN gene expression when compared to cells treated with higher concentrations of this hormone (10(-10) and 10(-8)M) or with the control group.
Conclusions:
Our data demonstrate that the increase in C-FOS and C-JUN expression and cell growth in HNTEP cells is maximal with the lowest DHT concentration (10(-13)M). These proto-oncogenes may play a role in the control of hormone responsiveness and cell proliferation in HNTEP cells.
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