Related Experiment Videos
Experimental autoimmune encephalomyelitis in cynomolgus monkeys.
1Department of Internal Medicine, University of Iowa, Iowa City, IA, 52242 USA. ki-hoan-nam@uiowa.edu
Journal of Veterinary Science
|November 14, 2003
Summary
Researchers induced experimental autoimmune encephalomyelitis (EAE) in macaques, observing distinct T cell populations in brain lesions compared to blood. Single-strand conformation polymorphism analysis of T cell receptor beta chains proved useful for studying T cell clonality in EAE brain tissue.
Area of Science:
- Neuroimmunology
- Primate Models
- Autoimmune Diseases
Background:
- Experimental autoimmune encephalomyelitis (EAE) is a model for human demyelinating diseases.
- Understanding T cell responses in the central nervous system is crucial for EAE pathogenesis.
Purpose of the Study:
- To investigate T cell infiltration and clonality in the brain lesions of macaques with EAE.
- To evaluate the utility of single-strand conformation polymorphism (SSCP) analysis for studying T cell populations in EAE.
Main Methods:
- EAE was induced in macaques via intradermal immunization with brain white matter and pertussis toxin.
- Neurological symptoms, gross brain examination, hematological analysis, flow cytometry (CD4, CD8, CD69), and SSCP analysis of T cell receptor beta chains were performed.
Main Results:
- Immunization induced neurological symptoms and hemorrhagic brain lesions in macaques.
- Significant T cell responses, including increased CD4+ and CD8+ T cells expressing CD69, were observed.
- SSCP analysis revealed distinct T cell clones in brain lesions compared to peripheral blood.
Conclusions:
- SSCP analysis of T cell receptor beta chains is a valuable method for assessing T cell clonality in primate EAE brain tissue.
- The findings highlight differences in T cell populations between the central nervous system and peripheral blood during EAE.