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Updated: Aug 30, 2026

Generation of Genetically Modified Mice through the Microinjection of Oocytes
Published on: June 15, 2017
Specific genetic modifications of domestic animals by gene targeting and animal cloning
1Nexia Biotechnologies Inc, 1000, Ave, St-Charles, Vaudreuil Dorion, Quebec, Canada, J7V 8P5. bwang@nexiabiotech.com
Abstract:
The technology of gene targeting through homologous recombination has been extremely useful for elucidating gene functions in mice. The application of this technology was thought impossible in the large livestock species until the successful creation of the first mammalian clone "Dolly" the sheep. The combination of the technologies for gene targeting of somatic cells with those of animal cloning made it possible to introduce specific genetic mutations into domestic animals. In this review, the principles of gene targeting in somatic cells and the challenges of nuclear transfer using gene-targeted cells are discussed. The relevance of gene targeting in domestic animals for applications in bio-medicine and agriculture are also examined.
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