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Updated: Aug 30, 2026
Generation of Immature, Mature and Tolerogenic Dendritic Cells with Differing Metabolic Phenotypes
Published on: June 22, 2016
Development of a new protocol for 2-day generation of mature dendritic cells from human monocytes
Bianca Obermaier1, Marc Dauer, Jan Herten
1Division of Clinical Pharmacology, Medizinische Klinik Innenstadt, University of Munich. Ziemssenstr. 1, 80336 Munich. Germany. B.O. and M.D. contributed equally to this manuscript.
Abstract:
We developed a new 2-day protocol for the generation of dendritic cells (DCs) from human monocytes in vitro. First, we demonstrated that 24 hours of culture with GM-CSF and IL-4 are sufficient to generate immature DCs capable of antigen uptake. We then compared two different strategies for DC maturation: proinflammatory mediators were either added together with GM-CSF and IL-4 from the beginning of cell culture or added after 24 hours of differentiation with GM-CSF and IL-4. After 48 hours of total culture period, expression of activation markers was more pronounced in cells generated by the 2-step differentiation and activation method. Our new protocol for 2-day DC differentiation reduces labor, cost and time and also reliably renders high numbers of mature and viable DCs.
