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Updated: Aug 30, 2026

Isolation and Culture of Primary Mouse Keratinocytes from Neonatal and Adult Mouse Skin
Published on: July 14, 2017
Expression of nitric oxide synthases in keratinocytes after UVB irradiation
Huoy-Rou Chang1, Der-An Tsao, Shiow-Ru Wang
1Department of Biomedical Engineering, I-Shou University, Section 1, 1 Shiuecheng Road, Dashu Shiang, Kaohsiung County, Taiwan. huoy@isu.edu.tw
Abstract:
The importance of nitric oxide (NO) in mediating vasodilation, neurotransmission, and immune and inflammatory responses has been demonstrated. Human keratinocyte express inducible nitric oxide synthase (iNOS) and the neuronal constitutive isoform of NOS (ncNOS). We established an in vitro model in keratinocytes to investigate changes in NO, iNOS and ncNOS expression after UVB exposure. We demonstrated a large induction of NO after UVB exposure and that the source of NO produced in UVB-exposed keratinocytes was increased expression of iNOS and ncNOS. The increased NO production with increased expression of iNOS and ncNOS may contribute to the pathological and physiological features of UVB-induced erythema and skin inflammation.
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