Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Yeast vacuole inheritance and dynamics.

Lois S Weisman1

  • 1Department of Biochemistry, University of Iowa, Iowa City, Iowa 52242, USA. lois-weisman@uiowa.edu

Annual Review of Genetics
|November 18, 2003
PubMed
Summary

Budding yeast Saccharomyces cerevisiae actively divides its vacuole/lysosome during cell division. This organelle inheritance involves a unique segregation structure, ensuring content exchange between mother and daughter cells.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dual endomembrane recycling pathways function in parallel to support synapse maintenance and plasticity.

Neuroscience·2025
Same author

PI(3)P coordinates SNX17- and SNX27-dependent protein recycling for long-term synaptic plasticity.

The Journal of cell biology·2025
Same author

Editorial Overview - Membrane traffic; orchestrating the symphony of life.

Current opinion in cell biology·2025
Same author

PIKFYVE-dependent regulation of MTORC1 and TFEB.

Autophagy reports·2025
Same author

Cargo adaptors use a handhold mechanism to engage with myosin V for organelle transport.

The Journal of cell biology·2025
Same author

VAC14 oligomerization is essential for the function of the FAB1/PIKfyve-VAC14-FIG4 complex.

Molecular biology of the cell·2025

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Yeast Genetics

Background:

  • The vacuole/lysosome is a crucial organelle in yeast, actively partitioned during cell division.
  • Vacuole inheritance occurs early in the cell cycle, preceding nuclear migration.

Purpose of the Study:

  • To elucidate the molecular mechanisms underlying vacuole inheritance in Saccharomyces cerevisiae.
  • To understand the dynamics of organelle segregation and content exchange during budding.

Main Methods:

  • Genetic analyses to identify key genes involved in vacuole division.
  • Biochemical assays to study protein interactions and membrane dynamics.
  • Cytological observations to visualize vacuole morphology and segregation structures.

Main Results:

  • Identified a tubular-vesicular segregation structure essential for vacuole inheritance.
  • Demonstrated the role of this structure in maintaining content exchange between mother and daughter vacuoles.
  • Provided insights into the spatial and temporal regulation of organelle transport.

Conclusions:

  • Vacuole inheritance in yeast is a tightly regulated process involving specialized structures.
  • The study sheds light on the molecular basis of membrane fusion, fission, and transport during organelle division.

Related Experiment Videos