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Characterization of allergens of Anisakis simplex
L G Arlian1, M S Morgan, S Quirce
1Department of Biological Sciences, Wright State University, Dayton, OH 45435, USA.
Allergy
|November 18, 2003
Summary
Anisakis simplex (L3) larvae contain numerous allergens that trigger IgE-mediated allergic reactions in humans. Patient responses to these allergens vary significantly, with no single allergen recognized by all individuals.
Area of Science:
- Parasitology
- Immunology
- Food Allergy
Background:
- Anisakis simplex is a marine parasite whose larvae (L3) infect fish.
- Human consumption of raw or undercooked fish can lead to Anisakis simplex infection.
- The parasite can cause inflammation and IgE-mediated allergic reactions due to its proteins.
Purpose of the Study:
- To investigate the allergenicity of proteins from Anisakis simplex third stage larvae (L3).
Main Methods:
- Crossed immunoelectrophoresis (CIE) was used to analyze somatic extracts of A. simplex L3.
- Crossed radioimmunoelectrophoresis (CRIE) identified IgE-binding proteins using sera from allergic individuals.
Main Results:
- CIE revealed 12-16 antigen-antibody precipitin peaks.
- CRIE identified 18 distinct proteins in the extract that bind patient IgE.
- Individual patients showed IgE reactivity to 2-10 different allergens, with 5 allergens recognized by over 50% of participants.
Conclusions:
- Anisakis simplex L3 somatic extracts contain multiple allergenic molecules.
- Significant patient variability exists in sensitivity and reactivity to these Anisakis allergens.
