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Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival Material
Published on: August 1, 2018
DNA amplification at 11q13.5-q14 in human breast cancer
P Szepetowski1, V Ollendorff, J Grosgeorge
1LGMCH, CNRS URA 1462, Faculté de Médecine, Nice, France.
Oncogene
|December 1, 1992
Summary
The D11S833E gene on chromosome 11q13.5-q14 represents a new, independent amplification unit in breast cancer. This finding helps explain complex amplification events in human breast tumors.
Area of Science:
- Genetics
- Oncology
- Molecular Biology
Background:
- Chromosome 11q13 is frequently amplified in human breast cancers.
- The specific genes driving these amplifications remain largely unknown.
- Previous studies focused on the BCL1 locus within 11q13.
Purpose of the Study:
- To investigate a more telomeric region at 11q13.5-q14.
- To identify new amplification units in breast cancer.
- To characterize the D11S833E transcription unit and its amplification patterns.
Main Methods:
- Analysis of DNA from breast cancer cell lines and primary tumors.
- Use of molecular probes to detect gene amplification.
- Mapping of the KRN1 gene relative to BCL1 and D11S833E.
Main Results:
- Amplicons encompassing both BCL1 and D11S833E showed interruptions between these loci.
- The KRN1 gene was absent in 40% of these interrupted amplicons.
- D11S833E was found amplified in tumors independently of other known 11q13 markers.
Conclusions:
- D11S833E defines a novel and independent amplification unit in the 11q13.5-q14 region.
- This finding contributes to understanding the complexity of 11q13 amplifications in breast cancer.
- D11S833E may play a significant role in breast tumorigenesis.
