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[Preliminary research for constructing tissue-engineered oral mucosa].

Qinggao Song1, Bing Shi

  • 1Department of Oral and Maxillofacial Surgery, West China School of Stomatology, Sichuan University, Chengdu 610041, China.

Sichuan Da Xue Xue Bao. Yi Xue Ban = Journal of Sichuan University. Medical Science Edition
|November 19, 2003
PubMed
Summary

Researchers developed a method for tissue-engineered oral mucosa using cultured oral keratinocytes and a sodium alginate membrane. This technique successfully created functional oral mucosa, offering potential for regenerative medicine applications.

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Area of Science:

  • Regenerative Medicine
  • Biomaterials Science
  • Tissue Engineering

Background:

  • Oral mucosa defects can result from trauma, surgery, or disease.
  • Current treatments for oral mucosa reconstruction have limitations.
  • Tissue engineering offers a promising alternative for creating functional oral mucosa grafts.

Purpose of the Study:

  • To establish a reproducible method for constructing tissue-engineered oral mucosa.
  • To evaluate the efficacy of cultured oral keratinocytes and sodium alginate membranes in oral mucosa regeneration.

Main Methods:

  • Rat hard palate mucoperiosteum was harvested.
  • Oral keratinocytes were isolated using Dispase digestion.
  • Cells were cultured in serum-free keratinocyte medium.

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  • A supportive membrane was fabricated from sodium alginate.

    • Main Results:

    • Dispase digestion effectively isolated rat oral mucosal epithelial cells.
    • Optimal Dispase digestion time was found to be ten minutes.
    • The ideal cell density for culture was 1.5 x 10(5)/cm2 to ensure proper cell colonization.
    • Keratinocytes cultured in serum-free medium showed no fibroblast contamination.
    • Satisfactory growth of mucosa cells was observed on the sodium alginate membrane.

    Conclusions:

    • Tissue-engineered oral mucosa can be successfully constructed using cultured oral keratinocytes.
    • Serum-free keratinocyte medium supports cell growth and prevents contamination.
    • Self-made sodium alginate membranes serve as effective scaffolds for oral mucosa engineering.