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Updated: Aug 30, 2026

Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification
Published on: September 21, 2011
Choice of procedures for preparative chromatography
Oleg A Pisarev1, Natalia V Glasova
1Department of Biopolymers, Institute of Macromolecular Compounds, Russian Academy of Science, Bolshoi pr. 31, St. Petersburg 199004, Russia. pisarev@imc.macro.ru
Abstract:
Quantitative criteria, necessary and sufficient, were developed basing on the analysis of asymtotic solutions of border equations in elution sorption dynamics for realization of selectivity inversion for components in a chromatographic system due to the effect of kinetic selectivity. Analysis is suggested for experiments, where the new approach to realization of chromatographic processes using effect of kinetic selectivity allows optimization of preparative separation of biologically active substances. The approach suggested implies shortening of the experiment duration for separation processes, which can be crucial in most systems where components to be isolated are labile, or the process economics suffers considerably due to mobile phase, or energy consumption.
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