Related Experiment Video
Updated: Aug 30, 2026

DNA-affinity-purified Chip (DAP-chip) Method to Determine Gene Targets for Bacterial Two component Regulatory Systems
Published on: July 21, 2014
Sub-classification of response regulators using the surface characteristics of their receiver domains
Douglas J Kojetin1, Richele J Thompson, John Cavanagh
1Department of Molecular and Structural Biochemistry, North Carolina State University, 128 Polk Hall, Campus Box 7622, Raleigh, NC 27695, USA.
Abstract:
The omnipresent bacterial switch known as a two-component system is comprised of a response regulator and a sensor kinase with which it interacts. Sensor kinases have been classified and further sub-classified into groups based on their sequence similarity, loop lengths and domain organization. Response regulators have been classified predominantly by the identity and function of their output domains. Here, comparative based homology modeling of the receiver domains of the OmpR sub-family of response regulators in Bacillus subtilis and Escherichia coli suggests further sub-classification is possible. A color-coded scale is used to show trends in surface hydrophobicity. For the OmpR receiver domains modeled these trends allow further sub-classification. The specific surface regions used for this sub-classification procedure correlate with clusters of residues that are important for interaction with cognate four helix bundle HisKA/Hpt domains.
Related Concept Videos
Types of Receptors: Cell Surface Receptors
Transducer Mechanism: G Protein–Coupled Receptors
GPCRs are also called heptahelical, 7TM, or...
Global Regulatory Systems
Signal Transduction: Overview
Typically, signal transduction involves three...
G Protein-coupled Receptors
GPCRs are also called heptahelical, 7TM, or serpentine receptors, and consist of seven (H1-H7) transmembrane alpha-helices that span the bilayer to form a cylindrical core. The transmembrane helices are connected by three extracellular loops and three...
G Protein-coupled Receptors

