Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Yeast two-hybrid screens implicate DISC1 in brain development and function.

J Kirsty Millar1, Sheila Christie, David J Porteous

  • 1Medical Genetics Section, Department of Medical Sciences, The University of Edinburgh, Western General Hospital, Crewe Road, EH4 2XU, Edinburgh, UK. Kirsty.Millar@ed.ac.uk

Biochemical and Biophysical Research Communications
|November 19, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Methylome-wide association studies and epigenetic biomarker development for 133 mass spectrometry-assessed circulating proteins in 14,671 Generation Scotland participants.

Genome biology·2025
Same author

Effectiveness and Costs of Participant Recruitment Strategies to a Web-Based Population Cohort: Observational Study.

Journal of medical Internet research·2025
Same author

Polygenic prediction of body mass index and obesity through the life course and across ancestries.

Nature medicine·2025
Same author

Data Resource Profile: Whole-Blood DNA Methylation Resource in Generation Scotland (MeGS).

International journal of epidemiology·2025
Same author

Genome-wide characterization of 54 urinary metabolites reveals molecular impact of kidney function.

Nature communications·2025
Same author

Genome-wide association study of susceptibility to hospitalised respiratory infections.

Wellcome open research·2024
Same journal

NAT10-mediated RNA N4-acetylation promotes intestinal fibroblast senescence via DHRS2.

Biochemical and biophysical research communications·2026
Same journal

Deciphering potent MPL activation by the fucose-binding lectin thrombocorticin.

Biochemical and biophysical research communications·2026
Same journal

Mitochondrial genome alterations in cancer: From mutations and epigenetics to targeted therapiesack.

Biochemical and biophysical research communications·2026
Same journal

GPC3 chimeric antigen receptor (CAR)-NK cells combined with Enoblituzumab enhance the anti-tumor efficacy against hepatocellular carcinoma.

Biochemical and biophysical research communications·2026
Same journal

A miR-382-5p-PORCN axis modulates endogenous Wnt signaling during palatal development.

Biochemical and biophysical research communications·2026
Same journal

Development of a vitamin-related gene signature to predict the immune characteristics and prognosis of glioma.

Biochemical and biophysical research communications·2026
See all related articles

Disrupted-in-schizophrenia 1 (DISC1) interacts with 21 proteins involved in brain development and function. These findings offer new insights into the genetic basis of psychiatric disorders like schizophrenia.

Area of Science:

  • Neuroscience
  • Genetics
  • Molecular Biology

Background:

  • Disrupted-in-schizophrenia 1 (DISC1) is a key candidate gene implicated in the etiology of major psychiatric disorders, particularly schizophrenia.
  • DISC1 is known to localize to multiple subcellular compartments within neurons.

Purpose of the Study:

  • To identify novel DISC1-interacting proteins using yeast two-hybrid screens.
  • To elucidate the functional roles of DISC1 in central nervous system (CNS) development and function through its interaction partners.

Main Methods:

  • Yeast two-hybrid screens were performed using human fetal and adult brain cDNA libraries.
  • Proteomic analysis was conducted to identify proteins that interact with DISC1.

Main Results:

Related Experiment Videos

  • Twenty-one proteins were identified as potential DISC1 interactors.
  • These proteins are involved in diverse cellular functions, including gene transcription, mitochondrial function, actin cytoskeleton modulation, neuronal migration, glutamate transmission, and signal transduction.
  • One identified interactor, WKL1, has prior suggested links to catatonic schizophrenia.

Conclusions:

  • DISC1 interacts with a wide range of proteins, supporting its role in multiple cellular processes crucial for CNS development and function.
  • The identified interactors provide a basis for understanding DISC1's contribution to psychiatric illness pathogenesis.
  • Further investigation into DISC1-WKL1 interactions may illuminate specific mechanisms in schizophrenia etiology.