Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Beta-catenin regulates Cripto- and Wnt3-dependent gene expression programs in mouse axis and mesoderm formation.

Markus Morkel1, Joerg Huelsken, Maki Wakamiya

  • 1Max Delbrueck Center for Molecular Medicine, Robert-Roessle-Strasse 10, 13125 Berlin, Germany.

Development (Cambridge, England)
|November 19, 2003
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Human extrahepatic and intrahepatic cholangiocyte organoids show region-specific differentiation potential and model cystic fibrosis-related bile duct disease.

Scientific reports·2020
Same author

An organoid-derived bronchioalveolar model for SARS-CoV-2 infection of human alveolar type II-like cells.

The EMBO journal·2020
Same author

Author Correction: Fasting-mimicking diet and hormone therapy induce breast cancer regression.

Nature·2020
Same author

Establishment of human fetal hepatocyte organoids and CRISPR-Cas9-based gene knockin and knockout in organoid cultures from human liver.

Nature protocols·2020
Same author

Translation and Replication Dynamics of Single RNA Viruses.

Cell·2020
Same author

CRISPR-Cas Tools and Their Application in Genetic Engineering of Human Stem Cells and Organoids.

Cell stem cell·2020

Beta-catenin regulates gene expression during early embryonic development, controlling axis formation and mesoderm development. Cripto, a Nodal co-receptor, is a key target of beta-catenin signaling in both embryos and colon cancer.

Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Cancer Biology

Background:

  • Early embryonic development relies on intricate genetic networks.
  • Beta-catenin and Wnt signaling pathways are crucial for embryogenesis.
  • Dysregulation of these pathways is implicated in developmental disorders and cancer.

Purpose of the Study:

  • To characterize genetic networks regulating early embryonic development.
  • To identify genes regulated by beta-catenin during anteroposterior axis and mesoderm formation.
  • To investigate the role of Cripto as a beta-catenin target in development and cancer.

Main Methods:

  • Gene expression profiling in mutant mouse embryos (beta-catenin, Cripto, Wnt3).
  • Analysis of gene regulation by Wnt3/beta-catenin signaling.

Related Experiment Videos

  • Identification of primary targets of beta-catenin signaling.

    • Main Results:

    • Defined genes regulated by beta-catenin during anteroposterior axis and mesoderm formation.
    • Identified Cripto (Nodal co-receptor) as a primary beta-catenin target in embryogenesis and colon cancer.
    • Characterized gene groups regulated by Wnt3/beta-catenin during primitive streak and mesoderm formation.

    Conclusions:

    • Beta-catenin plays a pivotal role upstream of distinct gene expression programs.
    • These programs are essential for anteroposterior axis and mesoderm formation.
    • Cripto is a significant downstream effector of beta-catenin signaling in both development and colon carcinoma.