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Updated: Aug 30, 2026

DNAzyme 10-23 - Based Nanomachines for Nucleic Acid Recognition
Published on: February 9, 2024
Aryldiazomethanes for universal labeling of nucleic acids and analysis on DNA chips
Ali Laayoun1, Mitsuharu Kotera, Isabelle Sothier
1BioMérieux, Advanced Technology/Molecular Diagnostics, Marcy l'Etoile, France. ali.laayoun@eu.biomerieux.com
Abstract:
DNA and RNA labeling and detection are key steps in nucleic acid-based technologies, used in medical research and molecular diagnostics. We report here the synthesis, reactivity, and potential of a new type of labeling molecule, m-(N-Biotinoylamino)phenylmethyldiazomethane (m-BioPMDAM), that reacts selectively and efficiently with phosphates in nucleotide monomers, oligonucleotides, DNA, and RNA. This molecule contains a biotin as detectable unit and a diazomethyl function as reactive moiety. We demonstrate that this label fulfills the requirements of stability, solubility, reactivity, and selectivity for hybridization-based analysis and especially for detection on high-density DNA chips.
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