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[Establishment and identification of recombinant NS-1 cell strain that stably expresses chimeric HBc containing HBV
Ze-wei Tian1, Wen-qi Dong, Zhao-xia Liu
1Research Center for Hepatopathy of PLA, 458 Hospital of PLA, Guangzhou 510602, China.
Objective:
To establish recombinant NS-1 cell strain that is capable of stable expression of chimeric HBc particle containing HBV multi epitope short peptides.
Methods:
The recombinant plasmid, pHBc-Mep, was transfected into NS-1 cells via Lipofectamine, and the recombinant cell strain was screened with G418 and subclone screening. The expression products of the cells were examined by RT-PCR, ELISA, indirect immunofluorescence assay (IFA) and Western blotting.
Results:
The results of RT-PCR, ELISA, IFA and Western blotting demonstrated that the recombinant protein HBc-Mep was expressed in the screened cells after continuous cloning for 3 times, but not in cells transfected with pcDNA3.1 or nontransfected cells.
Conclusion:
The recombinant cell strain stably expressing chimeric HBc particle containing multi epitope short peptides of HBV, designated as NS/HBc-Mep, has been established successfully.
