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Updated: May 7, 2026
07:49
Isolating Stem Cells from Soft Musculoskeletal Tissues
Published on: July 6, 2010
Single hematopoietic stem cells generate skeletal muscle through myeloid intermediates
Fernando D Camargo1, Rahshaana Green, Yassemi Capetanaki
1Center for Cell and Gene Therapy, Baylor College of Medicine, One Baylor Plaza, BCM505, Houston, Texas 77030, USA.
Nature Medicine
|November 20, 2003
Summary
Bone marrow cells, specifically hematopoietic stem cells (HSCs), can form skeletal muscle fibers. Mature myeloid cells, not stem cells, contribute to muscle regeneration following injury and inflammation.
Area of Science:
- Cell Biology
- Hematology
- Muscle Regeneration
Background:
- Recent studies suggest bone marrow cells can differentiate into skeletal muscle.
- The precise cell types and mechanisms remain unclear, with some studies questioning the findings.
Purpose of the Study:
- To identify the cell types responsible for circulating myogenic activity in bone marrow.
- To elucidate the mechanisms by which bone marrow cells contribute to skeletal muscle regeneration.
Main Methods:
- Single CD45+ hematopoietic stem cell (HSC) transplantation.
- Lineage tracing strategies.
- Analysis of muscle regeneration and inflammatory cell infiltration.
Main Results:
- Circulating myogenic activity originates from HSCs and their progeny.
- Muscle regeneration and inflammation are essential for HSC contribution.
- Mature myeloid cells, not a myogenic stem cell intermediate, are the source of myofibers.
- Myeloid cells migrate to regenerating muscle and integrate into myofibers upon inflammatory cues.
Conclusions:
- Hematopoietic stem cells are the source of circulating myogenic activity.
- Mature myeloid cells contribute to skeletal muscle regeneration in response to injury and inflammation.
- The process involves inflammatory cues driving myeloid cell migration and incorporation into myofibers.
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