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Profiling of the cell surface proteome.

Jun Ho Jang1, Samir Hanash

  • 1University of Michigan, Department of Pediatrics, Ann Arbor, MI 48109, USA.

Proteomics
|November 20, 2003
PubMed
Summary
This summary is machine-generated.

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Comprehensive proteome analysis requires examining proteins within subcellular compartments. Cell surface proteome profiling reveals unexpected protein locations, offering new disease insights.

Area of Science:

  • Proteomics
  • Cell Biology
  • Biochemistry

Background:

  • Proteome mining requires analyzing proteins in subcellular compartments, including location, trafficking, and modifications.
  • The plasma membrane is crucial for diagnostics and therapeutics, housing cell surface proteins.
  • Current technologies enable comprehensive cell surface proteome profiling using protein tagging.

Purpose of the Study:

  • To investigate unexpected protein expression patterns on the cell surface.
  • To explore the diagnostic and therapeutic potential of cell surface and plasma membrane proteomes.

Main Methods:

  • Utilizing technologies for comprehensive cell surface proteome profiling.
  • Employing protein tagging methods on intact cells.

Related Experiment Videos

Main Results:

  • Emerging studies show proteins previously localized to other compartments, like the endoplasmic reticulum, are found on the cell surface.
  • Unexpected patterns of specific protein expression on the cell surface have been identified.

Conclusions:

  • Profiling cell surface and plasma membrane proteomes offers novel insights into protein localization.
  • This approach is likely to uncover disease-related alterations and advance diagnostics and therapeutics.