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Bile Duct Ligation in Mice: Induction of Inflammatory Liver Injury and Fibrosis by Obstructive Cholestasis
Published on: February 10, 2015
[Effect of dahuang zhechong pill on transforming growth factor-beta 1 in hepatic stellate cells in rats]
Lansheng Li1, Man Xu, Zhi-long Liu
1Department of Chinese Medicine, Zhuhai Municipal People's Hospital, 3rd Affiliated Hospital of Medical College of Jinan University, Guangdong 519000. lilansheng@21cn.com
Objective:
To explore the effect of Dahuang Zhechong Pill (DHZCP) on transforming growth factor-beta 1 (TGF-beta 1) in rats hepatic stellate cells.
Methods:
Liver fibrosis model rats were induced by CCl4 compound factor, and treated with DHZCP in three different dosages (ordinary, double and triple) separately. TGF-beta 1 and alpha-smooth muscle actin (alpha-SMA) synthesis expression, and grades of fibrosis were observed.
Results:
TGF-beta 1 and alpha-SMA expression in the group treated with ordinary dose of DHZCP was insignificantly different from those in the control group (P > 0.05), but the expression attenuated significantly after treatment with double or triple dose of DHZCP in the portal area and discontinuous fibrous septum (P < 0.05, P < 0.01).
Conclusion:
DHZCP of larger dosage could inhibit the hepatic stellate cells proliferation and secretion of TGF-beta 1, and reduce the genesis of collagen, so as to weaken the auto-secretion amplifying response of the cells, which might be one of the chief mechanisms of DHZCP in antagonizing liver fibrosis.
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