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A Protocol for Analyzing Hepatitis C Virus Replication
Published on: June 26, 2014
Refolding of a recombinant full-length non-structural (NS3) protein from hepatitis C virus by chromatographic
Ming Li1, Anton Poliakov, U Helena Danielson
1National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Science, PR China.
Biotechnology Letters
|November 25, 2003
Abstract:
The non-structural protein 3 (NS3) of Hepatitis C virus (HCV) was expressed as inactive aggregates in Escherichia coli. The protein was refolded by chromatographic techniques of which ion exchange chromatography was best for crude samples and gel filtration best for partially purified samples. Immobilized metal ion affinity chromatography showed intermediate performance. Gradient procedures enhanced the recovery of active NS3 protein.
