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Updated: Aug 30, 2026
Wild-type Blocking PCR Combined with Sanger Sequencing for Detection of Low-frequency Somatic Mutation
Published on: August 23, 2024
[Investigation of p53 gene mutations in keloids using PCR-SSCP]
Yong-bo Liu1, Jian-hua Gao, Hong-jie Duan
1Department of Plastic Surgery, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
Objective:
To detect gene mutations of p53 gene (exon 4-6) in fibroblasts.
Methods:
Samples of keloids were taken from 15 patients. The mutations of p53 gene were detected using polymerase chain reaction, the single-strand conformational polymorphism(SSCP) analysis and DNA sequencing.
Results:
Gene mutations in p53 gene exon 4, 5, and 6 were identified in all the patients with keloids.
Conclusion:
Gene mutations resulted in keloid p53 protein losing its functions of suppressing cell processes and conducting apoptosis.
