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[Expression of androgen receptor isoforms in normal human prostate]
Shu-Jie Xia1, Gang-Yue Hao, Wei Wen
1Department of Urology, Shanghai First People's Hospital, Jiaotong University, Shanghai 200080, China.
Zhonghua Nan Ke Xue = National Journal of Andrology
|November 25, 2003
Summary
Normal human prostate tissue expresses four androgen receptor (AR) isoforms. These distinct AR isoforms show varying expression patterns, impacting prostate biology.
Area of Science:
- Andrology
- Molecular Endocrinology
- Prostate Biology
Context:
- The androgen receptor (AR) plays a crucial role in prostate function and disease.
- Understanding AR isoform expression in normal prostate is fundamental for interpreting pathological changes.
Purpose:
- To investigate and characterize the expression of androgen receptor (AR) isoforms in normal human prostate tissue.
- To identify the specific AR isoforms present and their binding characteristics.
Summary:
- Four distinct androgen receptor (AR) isoforms were identified in 14 normal human prostate specimens using high-resolution isoelectric focusing.
- These isoforms, with isoelectric point values of 6.5, 6.0, 5.8, and 5.3, demonstrated specific binding to 3H-dihydrotestosterone (DHT).
- Binding was inhibited by excess DHT and testosterone, but not by progesterone, estradiol, or diethylstilbestrol, indicating AR specificity.
Impact:
- This study reveals the heterogeneity of AR expression in normal prostate tissue.
- The findings provide a baseline for understanding AR isoform roles in prostate health and disease.
- Characterizing AR isoforms is essential for developing targeted therapies for prostate conditions.