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Updated: Aug 30, 2026

Two-dimensional Gel Electrophoresis Coupled with Mass Spectrometry Methods for an Analysis of Human Pituitary Adenoma Tissue Proteome
Published on: April 2, 2018
[Experimental research on human spermatozoa membrane proteins by two-dimensional gel electrophoresis]
Hao-Fei Wang1, Zu-Qiong Xiang, Yi-Xin Wang
1Department of Urology, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025, China. wangblack@citiz.net
Objectives:
To analyze human spermatozoa membrane proteins by two-dimensional gel electrophoresis and to provide a basis for drawing the protein map of normal human spermatozoa membrane proteins.
Methods:
Spermatozoa were purified by Percoll density centrifugation, and spermatozoa membrane proteins were analyzed by two-dimensional gel electrophoresis using isoelectric focusing and polyacrylamide gel electrophoresis.
Results:
About 800 protein spots could be identified by the imaging analysis system. The molecular weight and isoelectric point of most proteins were 20,000 to 100,000 and 3.0 to 7.0 respectively.
Conclusions:
There are more than 800 types of proteins in the human spermatozoa membrane. The spermatozoa membrane protein can be identified with a certain precision by two-dimensional gel electrophoresis.
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