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Liver and serum lysosomal enzymes activity during zymosan-induced inflammation in mice

A F Safina1, T A Korolenko, G I Mynkina

  • 1Institute of Physiology, Academy of Medical Sciences of USSR, Novosibirsk.

Agents and Actions. Supplements
|January 1, 1992
PubMed

Insights

This study investigated lysosomal enzymes during inflammation. Zymosan stimulation increased macrophage activation markers and liver cysteine proteinases, indicating inflammation

Area of Science:

  • Immunology and Biochemistry

Background:

  • Lysosomal enzymes play crucial roles in cellular processes, including immune responses.
  • Macrophage activation is a key component of inflammation.

Purpose of the Study:

  • To investigate the activity of liver and serum lysosomal enzymes during zymosan-induced inflammation.
  • To examine the effects of macrophage depression on these enzymes.

Main Methods:

  • Studied lysosomal enzymes (acid glucosidases, cysteine proteinases) in liver and serum.
  • Used zymosan to induce inflammation in vivo.
  • Utilized Gadolinium chloride (GdCl3) to induce macrophage depression.

Main Results:

  • Zymosan-induced inflammation increased macrophage activation markers: beta-N-acetylglucosaminidase (NAGlu) and beta-N-acetylgalactosaminidase (NAGal) in liver and serum.
  • Enhanced activity of liver cysteine proteinases was observed during zymosan stimulation.
  • Similar, though less pronounced, changes were noted during GdCl3-induced macrophage depression.

Conclusions:

  • Lysosomal enzyme activity, particularly NAGlu and NAGal, reflects macrophage activation during inflammation.
  • Cysteine proteinases in the liver are upregulated during inflammatory responses.
  • GdCl3 partially mimics inflammatory effects on these enzymes, suggesting a role in modulating macrophage function.

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