Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mutation and single nucleotide polymorphism detection using temperature gradient capillary electrophoresis.

Kathleen M Murphy1, Karin D Berg

  • 1Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA. kmurphy4@jhmi.edu

Expert Review of Molecular Diagnostics
|November 25, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Culturally and trauma-informed music therapy: approaches for school settings illustrated through descriptive cases from Norway, the United States and South Africa.

Frontiers in psychiatry·2026
Same author

Impact of the Genius Digital Diagnostics System on workflow and accuracy compared with the ThinPrep Imaging System for review of ThinPrep Papanicolaou tests.

American journal of clinical pathology·2025
Same author

Screening Young Adult Cancer Patients for Vocational Rehabilitation Services: A Vocational Rehabilitation Readiness Screening Tool.

Journal of occupational rehabilitation·2025
Same author

The Penn Medicine COVID-19 Therapeutics Committee-Reflections on a Model for Rapid Evidence Review and Dynamic Practice Recommendations During a Public Health Emergency.

Open forum infectious diseases·2023
Same author

Factors contributing to financial distress in young adults with cancer: Material resources, health, and workplace.

Work (Reading, Mass.)·2023
Same author

Ancillary testing for melanoma: current trends and practical considerations.

Human pathology·2023
Same journal

Bacterial respiratory infections: advances in diagnostic strategies.

Expert review of molecular diagnostics·2026
Same journal

Artificial intelligence in molecular diagnostics for pandemic preparedness.

Expert review of molecular diagnostics·2026
Same journal

Navigating PD-L1 testing in immuno-oncology: analytical robustness, clinical validation, and the role of the VENTANA SP263 assay.

Expert review of molecular diagnostics·2026
Same journal

Extracellular vesicles as diagnostic and prognostic biomarkers in non-small cell lung cancer.

Expert review of molecular diagnostics·2026
Same journal

Explainable epigenetic aging clocks: an overview of existing AI models and approaches.

Expert review of molecular diagnostics·2026
Same journal

Neuro-axonal injury biomarker serum neurofilament light chain is associated with osteoarthritis: a dual-cohort study from NHANES and UK Biobank.

Expert review of molecular diagnostics·2026
See all related articles

Temperature gradient capillary electrophoresis (TGCE) offers a sensitive, high-throughput method for detecting DNA sequence alterations. This technology aids in identifying mutations and single nucleotide polymorphisms linked to human diseases.

Area of Science:

  • Genetics
  • Molecular Biology
  • Biotechnology

Background:

  • Accurate detection of DNA sequence alterations is crucial for understanding human diseases.
  • Existing mutation screening methods often lack sensitivity or require extensive optimization.
  • Direct gene sequencing is accurate but remains time-consuming and expensive for large-scale screening.

Purpose of the Study:

  • To review the utility of Temperature Gradient Capillary Electrophoresis (TGCE) for mutation detection.
  • To evaluate TGCE as a high-throughput screening technology for genetic variations.
  • To assess TGCE's effectiveness in identifying known and discovering novel sequence alterations.

Main Methods:

  • Utilizes the denaturing properties of temperature gradients.

Related Experiment Videos

  • Leverages the high-resolution separation power of capillary electrophoresis.
  • Detects heteroduplex formation between mutant and wild-type DNA sequences.

    • Main Results:

    • TGCE demonstrates high sensitivity in detecting sequence alterations.
    • The technology is suitable for high-throughput screening applications.
    • TGCE can be applied to both known mutation detection and de novo mutation discovery.

    Conclusions:

    • Temperature Gradient Capillary Electrophoresis is a valuable tool for rapid mutation screening.
    • TGCE offers an efficient alternative to traditional sequencing methods for identifying disease-related genetic variations.
    • Further application of TGCE can advance genetic research and diagnostics.