Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Fluorescent resolution target for super-resolution microscopy.

P R H Stark1, L J Rinko, D N Larson

  • 1Harvard Medical School Technology and Engineering Center, Boston, MA, USA.

Journal of Microscopy
|November 25, 2003
PubMed
Summary
This summary is machine-generated.

Researchers developed a new fluorescent resolution target to better characterize optical microscopy systems. This new target overcomes limitations of current bead-based methods for assessing super-resolution microscopy capabilities.

Related Experiment Videos

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Short order nanohole arrays in metals for highly sensitive probing of local indices of refraction as the basis for a highly multiplexed biosensor technology.

Methods (San Diego, Calif.)·2005
Same author

Toxicity of 3-methyleneoxindole, a proposed reactive intermediate in the metabolism of 3-methylindole.

Advances in experimental medicine and biology·1991
Same author

Bioactivation of 3-methylindole by isolated rabbit lung cells.

Toxicology and applied pharmacology·1990
Same journal

In operando imaging of the space-charge region in a 4H-SiC MOSCAP using STEM-EBIC.

Journal of microscopy·2026
Same journal

The future of DXA: How AI is transforming bone health diagnostics.

Journal of microscopy·2026
Same journal

The Origins of Ploem's Filter Cube: A Pandora's Box.

Journal of microscopy·2026
Same journal

The reproducibility gap in graph neural network workflows for cell dynamics: A checklist-driven case study.

Journal of microscopy·2026
Same journal

Assessing the reproducibility of a bioimage analysis workflow characterising tissue flow in Drosophila.

Journal of microscopy·2026
Same journal

Modular training resources for bioimage analysis.

Journal of microscopy·2026
See all related articles

Area of Science:

  • Optical Microscopy
  • Super-Resolution Imaging
  • Nanotechnology

Background:

  • The Rayleigh criterion traditionally limits resolution in fluorescence optical microscopy.
  • Recent advancements have enabled techniques surpassing the Rayleigh criterion, including 4-Pi confocal microscopy and stimulated emission depletion microscopy.
  • Current resolution testing using fluorescent beads does not fully demonstrate classical resolution.

Purpose of the Study:

  • To fabricate a novel fluorescent resolution target.
  • To enable better characterization of a system's resolution capabilities in super-resolution microscopy.
  • To provide a more accurate method for assessing classical resolution.

Main Methods:

  • Fabrication of a specialized fluorescent resolution target.
  • Utilizing the target for characterizing optical microscopy systems.
  • Comparison with existing bead-based resolution testing methods.

Main Results:

  • The fabricated target allows for more precise characterization of optical system resolution.
  • The new target provides a better assessment of classical resolution compared to bead arrays.
  • Demonstrated capability to evaluate systems achieving sub-Rayleigh criterion resolution.

Conclusions:

  • The developed fluorescent resolution target offers an improved method for characterizing optical microscopy resolution.
  • This tool is crucial for accurately evaluating the performance of advanced super-resolution techniques.
  • The target facilitates a more rigorous assessment of classical resolution in microscopy.