Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Circulating glycated albumin and glomerular anionic charges.

I Londoño1, D Gingras, M Bendayan

  • 1Department of Pathology and Cell Biology, Université de Montréal, Montreal, Quebec, Canada.

Experimental Diabesity Research
|November 25, 2003
PubMed
Summary

Circulating glycated albumin alters kidney filtration in diabetes. However, this study found that anionic charges in the glomerular basement membrane are not responsible for these early changes in glomerular permeability.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

COVID-19: semen impairment may not be related to the virus.

Human reproduction (Oxford, England)·2021
Same author

What could cause the long-term effects of COVID-19 on sperm parameters and male fertility?

QJM : monthly journal of the Association of Physicians·2021
Same author

Covid-19 and impairment of spermatogenesis: What if fever was the only cause?

EClinicalMedicine·2020
Same author

Predicting sperm extraction in non-obstructive azoospermia patients.

Human reproduction (Oxford, England)·2020
Same author

COVID-19 and andrology: Recommendations of the French-speaking society of andrology (Société d'Andrologie de langue Française SALF).

Basic and clinical andrology·2020
Same author

[Environment and lifestyle: Impacts on male fertility?]

Gynecologie, obstetrique, fertilite & senologie·2017

Area of Science:

  • Nephrology
  • Diabetic Nephropathy
  • Renal Physiology

Background:

  • Diabetes mellitus is a leading cause of chronic kidney disease.
  • Glomerular dysfunction, characterized by altered filtration, is a hallmark of diabetic nephropathy.
  • The role of circulating glycated albumin in initiating these changes is not fully understood.

Purpose of the Study:

  • To investigate the mechanisms by which circulating glycated albumin affects glomerular filtration properties.
  • To determine if alterations in anionic charge distribution in the glomerular wall contribute to diabetic glomerular dysfunction.
  • To examine the relationship between glycated albumin, glomerular permselectivity, and anionic charge density.

Main Methods:

  • Intravascular infusion of Amadori products (glycated albumin) into normoglycemic rats.

Related Experiment Videos

  • Utilized polylysine-gold as a probe to visualize and quantify anionic charge distribution in the glomerular basement membrane.
  • Morphometrical evaluation of gold labeling on renal tissue sections from injected, diabetic, and newborn rats.

    • Main Results:

    • Infusion of glycated albumin altered glomerular filtration properties in normal rats.
    • No significant changes were observed in the distribution or density of anionic charges in the glomerular basement membrane after glycated albumin infusion.
    • In contrast, diabetic and newborn rats with abnormal glomerular filtration showed altered glomerular anionic charge distribution.

    Conclusions:

    • Anionic charges within the glomerular basement membrane do not appear to be the primary factor responsible for the early alterations in glomerular permeability induced by circulating glycated albumin.
    • Changes in glomerular anionic charges are associated with established abnormal renal function in diabetic conditions.
    • Further research is needed to elucidate the specific mechanisms by which glycated albumin impacts glomerular filtration.