Related Experiment Video
Updated: Aug 16, 2026

A Lab-On-A-Chip Platform for Stimulating Osteocyte Mechanotransduction and Analyzing Functional Outcomes of Bone Remodeling
Published on: May 21, 2020
Study of labeled carbonate metabolism in healthy organisms during reparative osteogenesis and denervation by the
Yu A Petrovich1, R P Podorozhnaya, S M Kichenko
1Moscow State Medical and Stomatological University.
Abstract:
We studied the effect of mandibular fracture and denervation on [(14)C]carbonate metabolism. A new index (bone/plasma relative radioactivity) reflecting the ratio between (14)C incorporation into bone and plasma was proposed. The percentage of label incorporation and bone/plasma relative radioactivity were measured from the 5th minute to the 192nd hour after intraperitoneal injection of labeled carbonate to 1-2-month-old albino rats. We revealed a biphasic reaction: rapid accumulation and elimination of the isotope form the bone; and slow accumulation followed by slow accumulation and elimination. Changes in carbonate metabolism after bone fracture corresponded to phases of reparative osteogenesis. At the stage of cellular-and-fibrous callus accumulation of labeled carbonate dominated over its elimination. At the stage of chondroid callus elimination dominated over accumulation. At the stage of primary osseous callus elimination dominated over accumulation, but did not surpass the control level. After fracture the index of bone/plasma relative radioactivity underwent general changes that were most pronounced in the zone of trauma. After denervation this index also decreased.
Related Concept Videos
Bone Remodeling
Osteoclasts in Bone Remodeling
The Bone Matrix
Skeleton and Calcium Homeostasis
Bone Remodeling and Repair
Role of Vitamins in Maintaining Bone Health
Vitamin A
Vitamin A is involved in the process of bone remodeling. Retinoic acid, the active metabolite of Vitamin A, has nuclear receptors in osteoblasts and osteoclasts, which are involved in bone remodeling.
Vitamin B12
Vitamin B12 acts as a cofactor during the formation of osteoblast-related proteins, such as osteocalcin. Vitamin B12 plays a role...

