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Related Experiment Videos

Cross-reactive metal ion sensor array in a micro titer plate format.

Torsten Mayr1, Christian Igel, Gregor Liebsch

  • 1Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, D-93040 Regensburg, Germany.

Analytical Chemistry
|November 25, 2003
PubMed
Summary

This study introduces a novel cross-reactive array for detecting metal ions using fluorescence. The method enables simultaneous, sensitive analysis of multiple cations in a microtiter plate format.

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Area of Science:

  • Analytical Chemistry
  • Chemical Sensing
  • Spectroscopy

Background:

  • Microtiter plate (MTP) formats offer high-throughput screening capabilities.
  • Detecting multiple divalent cations simultaneously requires selective and sensitive analytical methods.
  • Existing methods may lack the flexibility for simultaneous interrogation of various analytes.

Purpose of the Study:

  • To develop a versatile and flexible cross-reactive array for metal ion detection.
  • To enable simultaneous interrogation and imaging of metal ion probes at identical wavelengths.
  • To utilize chemometric tools for analyzing complex fluorescence patterns generated by unspecific indicators.

Main Methods:

  • Utilized a cross-reactive array in a microtiter plate (MTP) format with unspecific metal ion probes.

Related Experiment Videos

  • Employed blue-light-emitting diodes (LEDs) and a CCD camera for probe excitation and imaging.
  • Applied dual lifetime referencing to convert fluorescence intensity into time-dependent parameters.
  • Analyzed probe response patterns using chemometric tools, including support vector machines.

    • Main Results:

    • Achieved simultaneous detection and imaging of probes in 96 wells using identical analytical wavelengths.
    • Demonstrated sensitive detection of dye concentrations in the nanomoles-per-liter range without amplification.
    • Support vector machines accurately predicted the presence of significant metal ion concentrations based on generated patterns.

    Conclusions:

    • The developed cross-reactive array provides a highly flexible and efficient platform for metal ion detection.
    • Dual lifetime referencing and pattern recognition algorithms enhance the accuracy and sensitivity of the analysis.
    • This approach facilitates high-throughput, simultaneous quantification of multiple divalent cations.