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Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS)
Published on: March 14, 2013
A fully automated LC/MS method development and quantification protocol targeting 52 carbamates, thiocarbamates, and
Kate Yu1, Jim Krol, Michael Balogh
1Waters Corporation, 34 Maple Street, Milford, Massachusetts 01757, USA. Kate_yu@waters.com
Analytical Chemistry
|November 25, 2003
Summary
A novel automated liquid chromatography-mass spectrometry (LC/MS) method simplifies the analysis of 52 carbamates, thiocarbamates, and phenylureas in water samples. This efficient protocol meets EPA requirements for environmental monitoring.
Area of Science:
- Environmental Chemistry
- Analytical Chemistry
- Mass Spectrometry
Background:
- Carbamates, thiocarbamates, and phenylureas are common environmental contaminants.
- Accurate quantification of these compounds in water is crucial for regulatory compliance and public health.
Purpose of the Study:
- To develop and validate a fully automated LC/MS method for the simultaneous determination of 52 target analytes.
- To assess the method's sensitivity, selectivity, and applicability to real-world water samples.
Main Methods:
- Utilized a Waters Alliance HT LC system and ZQ 2000 MS detector with MassLynx software.
- Employed direct injection with a reversed-phase C8 column and a binary gradient elution.
- Leveraged MS multichannel capabilities to reduce reliance on chromatographic resolution.
Main Results:
- Successfully quantified 46 out of 52 target analytes with high coefficients of determination (0.886–0.999).
- Achieved limits of detection ranging from 0.091 to 19.3 ng/mL, meeting EPA sensitivity requirements.
- Demonstrated minimal matrix effects and high analyte recoveries (within EPA acceptance range) in spiked drinking and wastewater samples.
Conclusions:
- The developed automated LC/MS protocol is a robust, sensitive, and selective method for analyzing carbamates, thiocarbamates, and phenylureas.
- The method's efficiency and direct injection approach make it suitable for routine environmental monitoring.
- This protocol provides reliable quantification in complex matrices like drinking water and wastewater.
