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Updated: Aug 30, 2026
Robust Ligature-Induced Model of Murine Periodontitis for the Evaluation of Oral Neutrophils
Published on: January 21, 2020
Direct evidence of collagenolysis in chronic periodontitis
Jian Ma1, Timo Sorsa, Clark R Billinghurst
1Anatomy, Biomedicum Helsinki, University of Helsinki, Finland.
Background:
There is no previous evidence that collagenases in chronic periodontitis excessively cleave collagen fibrils.
Objectives:
In this study the eventual presence of neoepitopes produced in such a cleavage were looked for.
Methods:
A polyclonal antibody, which recognizes collagenase-cleaved collagen type I 3/4 carboxy-terminal neoepitope (COL1-3/4C), was used in avidin-biotin-peroxidase complex staining.
Results:
In addition, moderate staining was seen in connective tissue bordering to the sulcular and junctional epithelium, surrounding some of the fibroblasts and in some areas infiltrated by inflammatory mononuclear cells. COL1-3/4C staining in chronic periodontitis was more extensive (6.3 +/- 1.2%, n = 10) and intense than that observed in controls (1.6 +/- 0.7%, n = 10, Unpaired Student's t-test, p < 0.01).
Conclusions:
It is concluded that collagenases produced by host cells contribute to periodontal tissue destruction and attachment loss.
