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Platelet storage solution improves the in vitro function of preserved platelet concentrate
K Karnicki1, C Johnson, J St Cyr
1ViaCell, LLC, Rochester, MN 55902, USA.
Vox Sanguinis
|November 25, 2003
Summary
ViaCyte preserves platelet function during storage, maintaining energy levels and aggregation capabilities. This experimental solution shows promise for reducing platelet lesions compared to standard citrate-dextrose-phosphate-plasma (CDP-P).
Area of Science:
- Biotechnology
- Hematology
- Biochemistry
Background:
- Stored platelets undergo biochemical changes, impairing hemostatic function, clot retraction, and wound healing.
- Current storage solutions like citrate-dextrose-phosphate-plasma (CDP-P) have limitations in preserving platelet viability.
Purpose of the Study:
- To evaluate the efficacy of ViaCyte, a novel preservative solution, in maintaining platelet function during storage.
- To compare ViaCyte's performance against the standard CDP-P storage solution.
Main Methods:
- Platelets were stored in either ViaCyte or CDP-P for 5 days at room temperature.
- Analysis included adenine nucleotide (ATP) levels, in vitro agonist-mediated P-selectin expression, and platelet aggregation.
Main Results:
- ViaCyte-stored platelets showed significantly lower resting P-selectin expression (12.2%) compared to CDP-P (44.4%) after 5 days.
- ViaCyte maintained ATP levels and platelet aggregation, whereas CDP-P storage led to depressed ATP and loss of aggregation.
- Activated P-selectin expression increased by 52% in ViaCyte-stored platelets versus only 2.5% in CDP-P stored platelets.
Conclusions:
- ViaCyte effectively protects platelets from storage-induced lesions, preserving their functional integrity.
- Platelets stored in ViaCyte retain energy levels and responsiveness to activation, ensuring better hemostatic potential.