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Related Experiment Videos

A new 9-lipoxygenase cDNA from developing rice seeds.

Kouichi Mizuno1, Toshii Iida, Atsushi Takano

  • 1Institute of Agricultural and Forest Engineering, University of Tsukuba, Tsukuba Science City, Ibaraki 305-8572, Japan. koumno@akita-pu.ac.jp

Plant & Cell Physiology
|November 25, 2003
PubMed
Summary

Researchers identified a new rice lipoxygenase (r9-LOX1) in developing seeds. This enzyme, similar to LOX-L3, shows C9-specific activity and is useful for synthesizing specific fatty acids.

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Oxylipin KODA enhances the early growth of rice (<i>Oryza sativa</i> L.) under low-temperature stress at night to simulate a natural temperature condition.

Plant biotechnology (Tokyo, Japan)·2025

Area of Science:

  • Plant biochemistry
  • Molecular biology
  • Enzymology

Background:

  • Lipoxygenases (LOX) are key enzymes in plant lipid metabolism, involved in producing signaling molecules and defense compounds.
  • Rice (Oryza sativa) possesses multiple LOX isozymes with varying substrate specificities and expression patterns.
  • Understanding specific LOX activities is crucial for manipulating fatty acid profiles in crops.

Purpose of the Study:

  • To isolate and characterize a novel C9-specific lipoxygenase from rice seeds.
  • To determine the gene's location and expression pattern.
  • To evaluate the potential of the isolated enzyme for synthesizing specific oxylipins.

Main Methods:

  • cDNA isolation from developing rice seeds.
  • Enzymatic activity assays to determine substrate specificity.

Related Experiment Videos

  • Homology search against rice nucleotide databases.
  • Southern blot analysis and restriction enzyme mapping for gene localization.
  • Quantitative analysis of gene expression in different seed stages.

    • Main Results:

    • A novel C9-specific lipoxygenase cDNA, designated r9-LOX1, was successfully isolated.
    • r9-LOX1 exhibits enzymatic characteristics similar to rice LOX-L3, with a preference for C9 oxygenation.
    • The gene encoding r9-LOX1 is located on rice chromosome 3.
    • Gene expression analysis revealed higher transcript levels in imbibed seeds compared to developing seeds.
    • The isolated enzyme demonstrated potential for the in vitro synthesis of 9,10-ketol-octadecadienoic acid.

    Conclusions:

    • The discovery of r9-LOX1 provides new insights into rice seed lipoxygenase diversity and function.
    • r9-LOX1's C9-specific activity and expression profile suggest roles in seed physiology, possibly during germination.
    • The enzyme's utility in synthesizing 9,10-ketol-octadecadienoic acid opens avenues for biotechnological applications in lipid chemistry.