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Isolation and culture of leukemia cell lines

Hans G Drexler1

  • 1DSMZ German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany.

Methods in Molecular Medicine
|November 25, 2003
PubMed
Abstract

No abstract available in PubMed .

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Cell Lines

A cell line is a population of cells grown in vitro that can be subcultured over several generations. Normal cells cease to divide after a certain number of cell divisions, a process known as replicative senescence. This number, called the Hayflick limit, was conceptualized by Leonard Hayflick in 1961 when he observed that fetal cells grown in culture could only divide 40-60 times. This limit is due to the shortening of the telomeres during each round of cell division, preventing cell division...