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Related Experiment Videos

Cell volume and bile acid excretion.

D Häussinger1, C Hallbrucker, N Saha

  • 1Medizinische Universitätsklinik, Freiburg, Federal Republic of Germany.

The Biochemical Journal
|December 1, 1992
PubMed
Summary

Liver cell volume significantly impacts bile flow and taurocholate excretion. Cell swelling stimulates taurocholate excretion, while shrinkage inhibits it, suggesting cell volume is a key regulator.

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[Not Available].

Zeitschrift fur Gastroenterologie·2015

Area of Science:

  • Hepatology
  • Cell Physiology
  • Biliary Excretion

Background:

  • Bile acid transport is crucial for liver function.
  • The role of hepatocyte volume in regulating bile acid excretion is not fully understood.

Purpose of the Study:

  • To investigate the relationship between liver cell volume and taurocholate excretion into bile.
  • To determine if cell volume changes influence bile flow and bile acid transport.

Main Methods:

  • Isolated perfused rat liver model.
  • Aniso-osmotic conditions and specific agents (amino acids, insulin, bile acids) were used to alter hepatocyte volume.
  • Taurocholate excretion, bile flow, and cell volume were measured.

Main Results:

  • Cell swelling, induced by hypo-osmotic conditions or amino acids, significantly stimulated taurocholate excretion and bile flow.
  • Cell shrinkage inhibited taurocholate excretion.
  • Taurocholate-induced cholestasis was observed during cell shrinkage.
  • Tauroursodeoxycholate increased cell volume and stimulated taurocholate excretion in a volume-dependent manner.

Conclusions:

  • Liver cell volume is a critical determinant of bile flow and biliary taurocholate excretion.
  • Swelling-induced stimulation of taurocholate excretion is likely independent of membrane potential changes.
  • Bile acids, such as tauroursodeoxycholate, can modulate hepatocyte volume, influencing taurocholate transport.
  • Taurocholate-induced cholestasis may be linked to cell volume changes.

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