Chimeric RNA/DNA oligonucleotide-directed gene targeting in rice
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Updated: Aug 8, 2026
Generation of RNA/DNA Hybrids in Genomic DNA by Transformation using RNA-containing Oligonucleotides
Published on: November 24, 2010
1Laboratory of Plant Breeding and Genetics, Graduate School of Agricultural Science, Tohoku University, Aoba-ku, 981-8555 Sendai, Japan.
Abstract:
Site-specific mutagenesis in a rice genome was obtained by introducing chimeric RNA/DNA oligonucleotides (COs) by means of particle bombardment. Three COs were designed to target the independent codons for Pro-171, Trp-548 and Ser-627 of the endogenous rice acetolactate synthase (ALS) gene so it would confer resistance to ALS-inhibiting herbicides. Sequencing of the ALS gene of herbicide-resistant plants demonstrated that the ALS sequence was modified in a site-specific fashion. The efficiency of gene conversion mediated by COs was estimated to be 1x10(-4). These results demonstrate that CO-directed gene targeting is feasible in rice.
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