Related Experiment Video

Updated: Aug 30, 2026

Assays for the Specific Growth Rate and Cell-binding Ability of Rotavirus
10:49

Assays for the Specific Growth Rate and Cell-binding Ability of Rotavirus

Published on: January 28, 2019

Genetic variability of human rotavirus strains isolated from Eastern and Northern India

Soma Das1, Vici Varghese, Susmita Chaudhuri

  • 1Division of Virology, National Institute of Cholera and Enteric Diseases, Kolkata, India.

Journal of Medical Virology
|November 25, 2003
PubMed

Insights

Genomic diversity of Group A rotavirus was studied in Indian children. G1P[8] was the predominant strain, with rare types and mixed infections also identified, indicating complex viral circulation.

Area of Science:

  • Virology
  • Epidemiology
  • Genetics

Background:

  • Rotavirus is a leading cause of severe diarrheal disease in young children globally.
  • Understanding the genomic diversity of rotavirus is crucial for vaccine development and public health strategies.

Purpose of the Study:

  • To determine the genomic diversity of Group A rotavirus strains in Eastern and Northern India.
  • To identify common and rare rotavirus genotypes circulating in the region.

Main Methods:

  • Epidemiological study involving collection of rotavirus-positive samples from children with diarrhea.
  • Genotyping of VP7 (G-type) and VP4 (P-type) genes using reverse transcription (RT) and multiplex PCR.
  • Sequencing of the VP7 gene for further analysis of specific strains.

Main Results:

  • G1P[8] was the predominant rotavirus strain (32.5%), followed by G2P[4] (4.7%).
  • Rare strains (e.g., G1P[6], G2P[8]) and mixed infections were detected in 14.3% and 30% of cases, respectively.
  • Two G9 strains with P[8] and P[19] specificities showed higher homology to international isolates, suggesting a distinct progenitor.

Conclusions:

  • The study identified a complex genomic diversity of rotavirus in India, with G1P[8] being the most common genotype.
  • The presence of rare strains and genetically distinct G9 strains highlights the need for continuous surveillance and potential impact on vaccine effectiveness.

More Related Videos

Simplified Reverse Genetics Method to Recover Recombinant Rotaviruses Expressing Reporter Proteins11:40

Simplified Reverse Genetics Method to Recover Recombinant Rotaviruses Expressing Reporter Proteins

Published on: April 17, 2020

Related Concept Videos

Viral Recombination00:57

Viral Recombination

Cells are sometimes infected by more than one virus at once. When two viruses disassemble to expose their genomes for replication in the same cell, similar regions of their genomes can pair together and exchange sequences in a process called recombination. Alternatively, viruses with segmented genomes can swap segments in a process called reassortment.
Viral Mutations00:36

Viral Mutations

A mutation is a change in the sequence of bases of DNA or RNA in a genome. Some mutations occur during replication of the genome due to errors made by the polymerase enzymes that replicate DNA or RNA. Unlike DNA polymerase, RNA polymerase is prone to errors because it is not capable of “proofreading” its work. Viruses with RNA-based genomes, like HIV, therefore accrue mutations faster than viruses with DNA-based genomes. Because mutation and recombination provide the raw material for adaptive...
Human Virome01:26

Human Virome

The human body harbors a vast and diverse viral community known as the human virome. The virome includes bacteriophages that infect bacteria, and eukaryotic viruses that infect human cells. Transient dietary and environmental viruses also contribute to this dynamic ecosystem. Estimates suggest the human body may contain on the order of 10¹³ viral particles, though abundance varies widely by body site and detection method.Comprehensive characterization of the virome has become possible only with...