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Related Experiment Video

Updated: Jul 10, 2026

Production of Apolipoprotein C-III Knockout Rabbits using Zinc Finger Nucleases
10:59

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Published on: November 18, 2013

Gene targeting in livestock.

A J Thomson1, M M Marques, J McWhir

  • 1Department of Gene Expression and Development, Roslin Institute, Roslin, Midlothian EH25 9PS, UK.

Reproduction (Cambridge, England). Supplement
|November 26, 2003
PubMed
Summary

Gene targeting in livestock enables precise genetic modifications for biotechnology applications like organ transplantation and protein production. Challenges remain in targeting non-expressed genes and improving cell viability for nuclear transfer.

Area of Science:

  • Biotechnology
  • Animal Science
  • Genetics

Background:

  • Nuclear transfer technology allows for genetic modification of livestock.
  • Gene targeting in livestock has advanced rapidly, enabling directed genetic changes.

Purpose of the Study:

  • To review the current state and potential applications of gene targeting in livestock.
  • To identify barriers hindering the broader application of this technology in agriculture.

Main Methods:

  • Review of existing literature and experimental data on gene targeting in livestock.
  • Analysis of successful and unsuccessful targeting experiments, focusing on gene expression and cell culture.

Main Results:

  • Gene targeting is feasible in livestock, with applications in xenotransplantation and recombinant protein production.

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  • Successful targeting has been limited to expressed genes in donor cell lines.
  • Difficulties exist in targeting non-expressed genes and maintaining the viability of targeted cells for nuclear transfer.

    • Conclusions:

    • Gene targeting in livestock holds significant promise for biotechnology and potentially agriculture.
    • Overcoming challenges in targeting non-expressed genes and improving cell culture protocols is crucial for future development.